铁皮石斛促生长内生真菌的筛选与鉴定(3)

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　　Screening and identification of endophytic fungi with growth promoting
　　effect on Dendrobium officinale
　　HOU Xiao-qiang1，2， GUO Shun-xing1*
　　（1.Institute of Medicinal Plant Development， Chinese Academy of Medical Sciences and
　　Peking Union Medical College， Beijing 100193， China；
　　2. College of Life Science， Langfang Normal University， Langfang 065000， China）
　　[Abstract] The endophytic fungi with plant growth promoting effects were screened by co-culture of each endophytic fungus and seedlings of Dendrobium officinale. Anatomical features of the inoculated roots were studied by paraffin sectioning. Morphological characteristics and rDNA ITS1-5.8S-ITS2 sequences were applied for the taxonomy of endophytic fungi. The results showed that 8 strains inoculated to D. officinale seedlings greatly enhanced plant height， stem diameter， new roots number and biomass. According to the anatomical features of the inoculated roots， each fungus could infect the velamina of seedlings. The hyphae or pelotons were existed in the exodermis passage cells and cortex cells. The effective fungi could not infect the endodermis and vascular bundle sheath， but which was exception for other fungi with harmful to seedlings. Combined with classic morphologic classification， 2 effective strains were identified which were subjected to Pestalotiopsis and Eurotium. Six species of fungi without conidiophore belonged to Pyrenochaeta， Coprinellus， Pholiota， Alternaria， Helotiales， which were identified by sequencing the PCR-amplified rDNA ITS1-5.8S-ITS2 regions. The co-culture technology of effective endophytic fungi and plant can apply to cultivate the seedlings of D. officinale. It is feasible to shorten growth cycle of D. officinale and increase the resource of Chinese herbs. (责任编辑：论文发表网)转贴于八度论文发表网: http://www.8dulw.com(论文网__代写代发论文_论文发表_毕业论文_免费论文范文网_论文格式_广东论文网_广州论文网)