不同真菌对2种药用石斛种子共生萌发的效应(4)

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　　Effects of different fungi on symbiotic seed germination of
　　two Dendrobium species
　　ZI Xiao-meng1， 2， GAO Jiang-yun1*
　　（1.Center for Integrative Conservation， Xishuangbanna Tropical Botanical Garden， Chinese
　　Academy of Sciences， Mengla 666303， China；

    2. University of Chinese Academy of Sciences， Beijing 100049， China）
　　[Abstract]The epiphytic orchid， Dendrobium aphyllum and D. devonianum are used as traditional Chinese medicine， and became locally endangered in recent years because of over-collection. We test the effect of inoculations of endophytic fungi FDaI7 （Tulasnella sp.）， FDd1 （Epulorhiza sp.） and FCb4 （Epulorhiza sp.）， which isolated from D. aphyllum， D. denonianum and Cymbidium mannii， respectively， on artificial substrate in these two Dendrobium species. In the symbiotic germination experiment， FDaI7 and FDd1 were effective for protocorm formation and seedling development of D. aphyllum and D. denonianum separately. After 60 days， 14.46% of the D. aphyllum seeds grown to protocorms and 12.07% developed to seedlings inoculated only with FDaI7， while contrasted with 0 when inoculated the other two isolates and non-inoculation treatment. However， in D. denonianum， seeds only grown to protocorms and developed to seedlings when inoculated with FDd1， the percentages were 44.36% and 42.91% distinguishingly. High specificity was shown in symbiotic germination on artificial substrate of Dendrobium. Protocorms could further develop to seedlings within or without light when inoculated the compatible fungi. However， light condition （12/12 h Light/Dark） produced the normal seedlings， while dark condition （0/24 h L/D） produced the abnormal seedlings. These may suggest that the development of young seedlings require light based on the effective symbiotic fungi. These findings will aid in seedling production of simulation-forestry ecology cultivation， conservation and reintroduction of Dendrobium. (责任编辑：论文发表网)转贴于八度论文发表网: http://www.8dulw.com(论文网__代写代发论文_论文发表_毕业论文_免费论文范文网_论文格式_广东论文网_广州论文网)