不同真菌对2种药用石斛种子共生萌发的效应(4)
[6] Oddie R L A, Dixon K W, Mc Comb J A. Influence of substrate on asymbiotic and symbiotic in vitro germination and seedling growth of two Australian terrestrial orchids [J]. Lindleyana, 1994, 9(3): 183.
[7] Johnson T R, Stewart S L, Dutra D,et al. Asymbiotic and symbiotic seed germination of Eulophia alta (Orchidaceae)-preliminary evidence for the symbiotic culture advantage [J]. Plant Cell Tissue Organ Cult, 2007, 90(3): 313.
[8] 高江云. 西双版纳石斛资源的保护利用[J]. 园艺学报, 1996, 23(2): 160.
[9] 王艳, 潘扬, 马国祥. 西双版纳地区的石斛资源调查及鉴定[J]. 中国野生植物资源, 1995 (3): 41.
[10] Zi X M, Sheng C L, Goodale U M, et al. In situ seed baiting to isolate germination-enhancing fungi for an epiphytic orchid, Dendrobium aphyllum (Orchidaceae)[J]. Mycorrhiza, 2014, doi:10.1007/s00572-014-0565-8..
[11] Seaton P, Pritchard H. Orchid seed stores for sustainable use: a model for future seed-banking activities[J]. Lankesteriana, 2011, 11(3): 349.
[12] 盛春玲, 李勇毅, 高江云. 硬叶兰种子的迁地共生萌发及有效共生真菌的分离和鉴定[J]. 植物生态学报, 2012, 36(8): 859.
[13] 白毓谦, 方善康, 高东, 等. 微生物实验技术[M], 济南: 山东大学出版社, 1987:57.
[14] Yagame T, Yamato M, Mii M, et al. Developmental processes of achlorophyllous orchid, Epipogium roseum: from seed germination to flowering under symbiotic cultivation with mycorrhizal fungus[J]. J Plant Res, 2007, 120(2): 229.
[15] Aewsakul N, Maneesorn D, Serivichyaswat P, et al. Ex vitro symbiotic seed germination of Spathoglottis plicata Blume on common orchid cultivation substrates[J]. Sci Hortic, 2013, 160: 238.
[16] Nontachaiyapoom S, Sasirat S, Manoch L. Isolation and identification of Rhizoctonia-like fungi from roots of three orchid genera, Paphiopedilum, Dendrobium, and Cymbidium, collected in Chiang Rai and Chiang Mai provinces of Thailand[J]. Mycorrhiza, 2010, 20(7): 459.
[17] Chen J, Wang H, Guo S X. Isolation and identification of endophytic and mycorrhizal fungi from seeds and roots of Dendrobium (Orchidaceae) [J]. Mycorrhiza, 2012, 22(4): 297.
[18] Roberts P. Rhizoctonia-forming fungi: a taxonomic guide[M]. London: Royal Botanic Gardens, Kew, 1999.
[19] Goh C J. Studies on flowering in orchids-A review and future directions[J]. Proceed Nagoya Int Orchid Show, 1992, 92: 44.
[20] Stewart S L, Kane M E. Symbiotic seed germination and evidence for in vitro mycobiont specificity in Spiranthes brevilabris (Orchidaceae) and its implications for species-level conservation[J]. In Vitro Cell Dev Biol Plant, 2007, 43(3): 178.
[21] Zettler L W, Hofer C J. Propagation of the little club-spur orchid (Platanthera clavellata) by symbiotic seed germination and its ecological implications[J]. Environ Exp Bot, 1998, 39(3): 189.
[22] Park E J, Lee W Y. In vitro symbiotic germination of myco-heterotrophic Gastrodia elata by Mycena species[J]. Plant Biotechnol Rep, 2013,7(2):185.
[23] 侯天文, 金辉, 刘红霞, 等. 实验室条件下五唇兰菌根真菌专一性研究[J]. 植物生态学报, 2010, 34(12): 1433.
[24] Steinfort U, Verdugo G, Besoain X, et al. Mycorrhizal association and symbiotic germination of the terrestrial orchid Bipinnula fimbriata (Poepp.) Johnst (Orchidaceae)[J]. Flora-Morphology, Distribution, Functional Ecology of Plants, 2010, 205(12):811.
[25] 吴慧凤, 宋希强, 刘红霞. 铁皮石斛种子的室内共生萌发[J]. 生态学报, 2012, 32(8): 2491.
[26] Wang H, Fang H, Wang Y, et al. In situ seed baiting techniques in Dendrobium officinale Kimuraet Migo and Dendrobium nobile Lindl.: the endangered Chinese endemic Dendrobium (Orchidaceae)[J]. World J Microbiol Biotechnol, 2011, 27(9): 2051.
Effects of different fungi on symbiotic seed germination of
two Dendrobium species
ZI Xiao-meng1, 2, GAO Jiang-yun1*
(1.Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, Chinese
Academy of Sciences, Mengla 666303, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China)
[Abstract]The epiphytic orchid, Dendrobium aphyllum and D. devonianum are used as traditional Chinese medicine, and became locally endangered in recent years because of over-collection. We test the effect of inoculations of endophytic fungi FDaI7 (Tulasnella sp.), FDd1 (Epulorhiza sp.) and FCb4 (Epulorhiza sp.), which isolated from D. aphyllum, D. denonianum and Cymbidium mannii, respectively, on artificial substrate in these two Dendrobium species. In the symbiotic germination experiment, FDaI7 and FDd1 were effective for protocorm formation and seedling development of D. aphyllum and D. denonianum separately. After 60 days, 14.46% of the D. aphyllum seeds grown to protocorms and 12.07% developed to seedlings inoculated only with FDaI7, while contrasted with 0 when inoculated the other two isolates and non-inoculation treatment. However, in D. denonianum, seeds only grown to protocorms and developed to seedlings when inoculated with FDd1, the percentages were 44.36% and 42.91% distinguishingly. High specificity was shown in symbiotic germination on artificial substrate of Dendrobium. Protocorms could further develop to seedlings within or without light when inoculated the compatible fungi. However, light condition (12/12 h Light/Dark) produced the normal seedlings, while dark condition (0/24 h L/D) produced the abnormal seedlings. These may suggest that the development of young seedlings require light based on the effective symbiotic fungi. These findings will aid in seedling production of simulation-forestry ecology cultivation, conservation and reintroduction of Dendrobium. (责任编辑:论文发表网)转贴于八度论文发表网: http://www.8dulw.com(论文网__代写代发论文_论文发表_毕业论文_免费论文范文网_论文格式_广东论文网_广州论文网)